Azai Nagamasa:
Defeat Musou Mode with Oichi

Shimazu Yoshihiro:
Defeat Musou Mode with Tachibana Ginchiyo

Honda Tadakatsu:
Defeat Musou Mode with Tokugawa Ieyasu or Have Samurai Warriors: Xtreme Legends save on memory card before starting the game

Hattori Hanzou:
Defeat Musou Mode with Fuuma Kotarou, or Have Samurai Warriors save on memory card before starting the game

Uesugi Kenshin:
Clear Musou Mode with Naoe Kanetsugu, or Have Samurai Warriors save on memory card before starting the game

Inahime:
Defeat Musou Mode with Honda Tadakatsu

Naoe Kanetsugu:
Defeat Musou Mode with Sanada Yukimura

Shima Sakon:
Defeat Musou Mode with Ishida Mitsunari

Maeda Keiji:
Defeat Musou Mode with Naoe Kanetsugu

Oda Nobunaga:
Defeat Musou Mode with Saiga Magoichi

Okuni:
Clear Sugoroku Mode (Single Play)

Saiga Magoichi:
Defeat Musou Mode with Akechi Mitsuhide

Takeda Shingen:
Defeat Musou Mode with Uesugi Kenshin

Date Masamune:
Defeat Musou Mode with Saiga Magoichi

Nouhime:
Defeat Musou Mode with Oda Nobunaga

Toyotomi Hideyoshi:
Defeat Musou Mode with Ishida Mitsunari

Nene:
Defeat Musou Mode with Toyotomi Hideyoshi

Mori Ranmaru:
Unlock him in Mugenjyou Mode

Miyamoto Musashi:
Unlock all other characters, not including Okuni and Ranmaru